FastQCFastQC Report
Tue 20 Jun 2023
HET-IgG_S27_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHET-IgG_S27_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36164823
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC700840.19379052401279553No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT445520.12319153338590928No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA427880.11831386538239107No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC369070.10205220691941448No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC362450.10022169885913725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)