FastQCFastQC Report
Tue 20 Jun 2023
HET-H3K4me3_S22_L004_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHET-H3K4me3_S22_L004_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24829827
Sequences flagged as poor quality0
Sequence length50-151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA3492001.4063730689706375No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC2376290.9570304295716598No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC2320930.9347346640796169No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT922310.3714524470911537No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG648940.2613550227313304No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG576880.23233347538023524No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG515270.2075205759589062No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA474830.1912337125828545No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT406030.1635251022892749No Hit
ACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAGGGAGTAA354120.14261879472619765No Hit
TAGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGC276200.11123718260300404No Hit
ACATCGGATCGACGCGTAAACGGTACGTCGGCCTGGAGACTAGGGAGTAA273090.10998465675979135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)