FastQCFastQC Report
Tue 20 Jun 2023
WT-H3K4me3_S17_L004_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT-H3K4me3_S17_L004_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33597231
Sequences flagged as poor quality0
Sequence length50-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA5296611.5765019444608397No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC4102151.2209785979088574No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC3987281.1867882802603584No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT1352390.40253019661054806No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1181630.35170457946370637No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA945660.2814696246842485No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG888890.2645723988384638No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT883300.2629085712450529No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG670690.19962657041587745No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT529080.15747726352805683No Hit
ACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAGGGAGTAA440600.13114175986705573No Hit
AGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCCTG382580.11387247955047249No Hit
TAGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGC356010.10596408971917953No Hit
AGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCTG347950.10356508249147081No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)