FastQCFastQC Report
Tue 20 Jun 2023
WT-H3K4me3_S18_L004_R2_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT-H3K4me3_S18_L004_R2_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30586567
Sequences flagged as poor quality0
Sequence length50-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA4569051.4938093575522875No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC4426461.4471908534226805No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC4181611.3671393719994793No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG2290920.7489954658853999No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT1196070.391044212317126No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1123310.3672559918215078No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG812990.2657996891249678No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA787540.25747904300603597No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG751130.24557512453097463No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT688340.22504650489216393No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT456080.14911120950579382No Hit
ACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAGGGAGTAA428450.14007783220653694No Hit
TAGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGC389920.127480799005655No Hit
AGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCCTG367600.1201834779300338No Hit
AGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCTG328510.10740335782044451No Hit
ACATCGGATCGACGCGTAAACGGTACGTCGGCCTGGAGACTAGGGAGTAA310100.10138437569669065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)