FastQCFastQC Report
Tue 20 Jun 2023
WT-IgG_S25_L004_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT-IgG_S25_L004_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35294530
Sequences flagged as poor quality0
Sequence length50-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC667530.18913129031609147No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT659480.18685048363018292No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG580650.16451557790966476No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA526780.14925258956557858No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC522390.1480087707641949No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC503980.14279266503903013No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC491690.13931053905520205No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG487940.13824805146859867No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCC462770.1311166347873169No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC451440.12790650562565928No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC429810.12177807722613108No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC423850.12008943028848945No Hit
CAAGGTCGCTGTTCAATACATCGGACGATCGTACGATAGCGTACCGAGCC420090.11902410940165516No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC416590.11803245432082535No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC415710.11778312390050244No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC413320.11710596514530722No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC410100.11619364247094378No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC381680.10814140321460577No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC376280.10661142108989692No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC374330.10605892754486318No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCC372910.10565659891206937No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCC371850.1053562690875895No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCC364770.10335029252408233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)