FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3E97K-HA-Clone19-aH3K4me3-2_S20_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3E97K-HA-Clone19-aH3K4me3-2_S20_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40144178
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC771800.1922570191871907No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC753310.18765112091720995No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTAGGGG659450.16427039557267806TruSeq Adapter, Index 2 (97% over 37bp)
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG636490.15855101080908918No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT426140.10615237905730687No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTGGGGG402900.1003632456990401TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)