FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3E97K-HA-Clone22-aH3K4me3-2_S28_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3E97K-HA-Clone22-aH3K4me3-2_S28_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27196364
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC1048680.3855956627143246No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC1008200.37071132008675867No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG871290.3203700318174886No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT612430.2251881905978314No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA519120.19087845713493173No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG341500.12556825610952993No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT282990.10405435079483419No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)