FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3E97K-HA-Clone22-aIgG-1_S25_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3E97K-HA-Clone22-aIgG-1_S25_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20421824
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG769580.3768419510421792No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG687680.3367377958011978No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG272750.1335580994136469No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT242380.11868675393539774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)