FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3E97K-HA-Clone23-aH3K4me3-2_S36_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3E97K-HA-Clone23-aH3K4me3-2_S36_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32936479
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG2870370.8714865969735259No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC2482560.753741770636746No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC2405780.7304302320840064No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT1716530.5211637831718442No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA1296250.39356058672816846No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG943420.2864362034569633No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA737480.22390978707833342No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT721000.21890621641736507No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG675100.20497030055944962TruSeq Adapter, Index 13 (97% over 38bp)
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT521290.15827131977282696No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG407640.12376550632506893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)