FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3WT-HA-aIgG-1_S9_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3WT-HA-aIgG-1_S9_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33986244
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG798030.23480970712738955No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG698450.20550961736165962No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGGG641930.18887935954323168TruSeq Adapter, Index 6 (97% over 36bp)
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT516700.15203209863378844No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTAGGGG443430.1304733762283352TruSeq Adapter, Index 6 (97% over 36bp)
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT366140.10773182232199593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)