FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3WT-HA-aIgG-2_S10_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3WT-HA-aIgG-2_S10_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29423854
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG789640.26836729138201953No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG779660.2649754855363271No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGGG567030.1927109888459887TruSeq Adapter, Index 6 (97% over 36bp)
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT485870.16512792647761235No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTAGGGG413550.14054922920702367TruSeq Adapter, Index 6 (97% over 36bp)
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT386690.1314205814098996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)