FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-Parental-aH3K4me3-2_S4_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-Parental-aH3K4me3-2_S4_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34536729
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC1771790.5130161573784247No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1706030.4939755586002369No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC1701390.49263206136284643No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT1158590.3354660483336451No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA1126370.3261368498446972No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTGGGGG1023000.29620639522636905TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA463500.13420495032983581No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT429980.12449934097696397No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT395760.11459104885120996No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG387530.11220807853575249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)