FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3E97K-HA-Clone19-aH3K4me3-2_S20_L008_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3E97K-HA-Clone19-aH3K4me3-2_S20_L008_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39243650
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length50-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC769540.19609287107595752No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC751420.19147556356251266No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG634030.16156244386034427No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTAGGGG560780.14289700371907302TruSeq Adapter, Index 2 (97% over 37bp)
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT424440.1081550773182413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)