FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3E97K-HA-Clone22-aH3K4me3-1_S27_L008_R2_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3E97K-HA-Clone22-aH3K4me3-1_S27_L008_R2_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23378916
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length50-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC1067310.45652672690213697No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC990740.4237749945292588No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG964850.4127009139345896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG889020.3802657060746529No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA515540.22051492892142646No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT514670.22014279875080608No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG337160.1442154118693955No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT247330.10579190241326844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)