FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3E97K-HA-Clone23-aIgG-1_S33_L008_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3E97K-HA-Clone23-aIgG-1_S33_L008_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26379633
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG958500.3633484969256396No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTGGGGG729030.27636093345195517TruSeq Adapter, Index 13 (97% over 38bp)
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG672680.2549997568199679No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT636980.24146658901585175No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT507510.19238705860691846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)