FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3WT-HA-aH3K4me3-1_S11_L008_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3WT-HA-aH3K4me3-1_S11_L008_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31816193
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length50-151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC2018100.634299647352529No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1942750.6106167384639639No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC1893120.5950177634388878No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT1426570.4483785976530882No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA860300.2703969013514596No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA659500.20728438502997515No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGGG530210.16664784501401536TruSeq Adapter, Index 6 (97% over 36bp)
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT496530.15606204048359903No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT400040.1257347162811088No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTAGGGG354000.1112640974990314TruSeq Adapter, Index 6 (97% over 36bp)
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG349710.10991572750391602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)