FastQCFastQC Report
Tue 2 Jan 2024
Beas2B-H3WT-HA-aIgG-2_S10_L008_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBeas2B-H3WT-HA-aIgG-2_S10_L008_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28866315
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG786440.27244211808815916No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG776950.2691545491691614No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGGG526670.18245141439078733TruSeq Adapter, Index 6 (97% over 36bp)
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT483780.16759326571472666No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT385130.13341848448615626No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTAGGGG366990.12713434326480536TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)