FastQCFastQC Report
Thu 11 May 2023
4a_S10_L002_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4a_S10_L002_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40287969
Sequences flagged as poor quality0
Sequence length50-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG555890.13797915700342203No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA512910.1273109597557524No Hit
CGCGAGCGAAGCCGTCGGGGGTGGGACAGGACCAGGCGGGACAGAGAGCG412830.10246979687658118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)