FastQCFastQC Report
Thu 21 Dec 2023
JX-4_S4_L003_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJX-4_S4_L003_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9573639
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG300130.3134962577970613No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAAC183540.19171393448196655No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG120990.12637827684958666No Hit
CGGAAAGAGAAGCGCGACACCGGCGAGACAGTCAAACCACGCGGGAGGGA103070.10766021154547398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)