FastQCFastQC Report
Sat 24 Jun 2023
EV-1205Lu-3_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEV-1205Lu-3_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28894761
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT724260.2506544352451989No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA390830.13525981405418097No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGTTTGATCTCGTAT329290.11396183550367488TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)