FastQCFastQC Report
Sat 24 Jun 2023
HDAC8-SKMEL28-1_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHDAC8-SKMEL28-1_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47333343
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGCGATATCTCGTAT2003260.4232238572289305TruSeq Adapter, Index 5 (97% over 37bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1448420.30600416285830473No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1077570.22765558730977442No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGCGATATCGCGTAT910520.19236334099621907TruSeq Adapter, Index 5 (97% over 37bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC798770.16875419088822863No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT564780.11931969394175265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)