FastQCFastQC Report
Sat 24 Jun 2023
EV-SKMEL28-3_S9_L001_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEV-SKMEL28-3_S9_L001_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36612774
Sequences flagged as poor quality0
Sequence length50-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA967690.2643039284595043No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT905990.24745188660110812No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC530750.1449630667154584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)