FastQCFastQC Report
Sat 24 Jun 2023
HDAC8-1205Lu-2_S5_L001_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHDAC8-1205Lu-2_S5_L001_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29995342
Sequences flagged as poor quality0
Sequence length50-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT774220.25811340974208596No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA445740.14860307310381723No Hit
GGAGAGGCGACGGGAGAGAGAGCCGCGGCCGACGGCACCCCCGCGCCGCC334920.11165733666247246No Hit
CAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGCCT310320.10345606327809165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)