FastQCFastQC Report
Tue 13 Jun 2023
D1240A-H3K27me3-Rep1_S11_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD1240A-H3K27me3-Rep1_S11_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34395801
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGTT794310.23093225827187452TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGGT751100.21836967832207194TruSeq Adapter, Index 6 (97% over 36bp)
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA594640.17288156772392071No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGGT444980.1293704426304827TruSeq Adapter, Index 6 (97% over 36bp)
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC370660.10776315399661719No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC360910.10492850566265342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)