FastQCFastQC Report
Tue 13 Jun 2023
D1240A-H3K4me3_S10_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD1240A-H3K4me3_S10_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34366992
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA1598990.46526911636607593No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGTT1391380.4048594069565355TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGGT1317050.38323109569787195TruSeq Adapter, Index 6 (97% over 36bp)
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC977560.28444735576508995No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC970850.28249490092120955No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGGT628770.18295753087730227TruSeq Adapter, Index 6 (97% over 36bp)
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG522440.1520179595584042No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT454740.13231882499347047No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG378590.1101609358188811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)