FastQCFastQC Report
Tue 13 Jun 2023
D1240A-IgG_S9_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD1240A-IgG_S9_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34113426
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGTT2017650.591453347429836TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGGT1898160.5564260827980162TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGGT1096380.3213925215251027TruSeq Adapter, Index 6 (97% over 36bp)
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG876400.2569076468602128No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGTT604100.17708570226866102TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTTT372060.10906556263214373TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)