FastQCFastQC Report
Tue 13 Jun 2023
F1295A-H3K27me3-Rep2_S16_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameF1295A-H3K27me3-Rep2_S16_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25093312
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA606680.2417695998041231No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGTT401880.1601542275487588TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGGT385430.1535986959393802TruSeq Adapter, Index 6 (97% over 36bp)
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC373430.14881654522129242No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC372520.14845389879183743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)