FastQCFastQC Report
Tue 13 Jun 2023
F1295A-IgG_S13_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameF1295A-IgG_S13_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33533122
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG1821190.5431018322719847No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGGT871180.25979686591663015TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGTT853020.25438132482862763TruSeq Adapter, Index 6 (97% over 36bp)
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC702220.20941086248992863No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC624720.18629938482912506No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGGT489300.14591543250878938TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)