FastQCFastQC Report
Tue 13 Jun 2023
WT-H3K27me3-Rep1_S7_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT-H3K27me3-Rep1_S7_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28948570
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA848450.2930887432436214No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTT795420.27477004909050773TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTT658150.2273514719379921TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT646630.2233720007585867TruSeq Adapter, Index 27 (97% over 39bp)
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC521660.18020233814658204No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC516610.1784578651035267No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGAT329690.1138881816960216TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)