FastQCFastQC Report
Tue 13 Jun 2023
WT-H3K27me3-Rep2_S8_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT-H3K27me3-Rep2_S8_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28508992
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA715050.2508156023194366No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTT494470.17344352266120108TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTT481220.1687958662305563TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT454260.15933920076865574TruSeq Adapter, Index 27 (97% over 39bp)
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC388010.13610091861543192No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC378680.13282826695521188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)