FastQCFastQC Report
Tue 13 Jun 2023
WT-IgG_S5_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT-IgG_S5_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22336225
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTT2021390.904982824984974TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTT1790180.8014693619893246TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1731660.775269769175409TruSeq Adapter, Index 27 (97% over 39bp)
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG895270.4008152675754296No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGAT815760.36521838403758916TruSeq Adapter, Index 27 (97% over 39bp)
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC638690.28594357372384993No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGGT432310.19354658184182869TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGGTT409360.18327179279399272TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTAT336540.15067004384133845TruSeq Adapter, Index 27 (97% over 39bp)
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT304980.1365405300134647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTTT294530.13186203129669405TruSeq Adapter, Index 27 (97% over 39bp)
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC286440.12824011219442855No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC265570.11889654585768186No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC263990.11818917475983519No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC261320.11699380714511964No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCC251500.11259736146103472No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC245340.10983950958588572No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC243510.10902021268141773No Hit
CAAGGTCGCTGTTCAATACATCGGATCGCGATTACGATGTCGCGCGAGCC235100.10525502854667698No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC231960.10384924041551337No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC229440.10272102828477059No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC228270.10219721550978288No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGGAT226200.10127046983095846TruSeq Adapter, Index 27 (97% over 39bp)
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC224800.10064368531387913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)