FastQCFastQC Report
Tue 13 Jun 2023
TKO-H3K4me3_S2_L004_R2_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTKO-H3K4me3_S2_L004_R2_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14646303
Sequences flagged as poor quality0
Sequence length50-151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG1233860.8424378493330364No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA1210730.826645468143053No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC968310.6611292965876782No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC930680.6354368061346266No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT347740.23742510311305182No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG301100.20558088959377668No Hit
ACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAGGGAGTAA197410.13478486687049968No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG160820.10980245321976474No Hit
ACATCGGATCGACGCGTAAACGGTACGTCGGCCTGGAGACTAGGGAGTAA152450.10408770049342828No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG149970.10239444042636561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)