FastQCFastQC Report
Mon 19 Jun 2023
RCH-ACV-2C-H3K4me3_S22_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRCH-ACV-2C-H3K4me3_S22_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31651117
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA6612432.089161655811389No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC4643821.467189925714154No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC4502761.4226227782103236No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT1652030.5219499836293298No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1390160.4392135670914868No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG1060640.33510349729521394No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA1032660.32626336694531194No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT961610.30381550199318397No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT854550.2699904714263323TruSeq Adapter, Index 7 (97% over 35bp)
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT603570.1906946917544806No Hit
ACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAGGGAGTAA584990.18482444079303742No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG584650.18471701962366763No Hit
ACATCGGATCGACGCGTAAACGGTACGTCGGCCTGGAGACTAGGGAGTAA426970.1348988726053491No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG357360.11290596789996384No Hit
TAGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGC355370.11227723811453479No Hit
AGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCTG328970.10393630025758649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)