FastQCFastQC Report
Mon 19 Jun 2023
RCH-ACV-9B-IgG_S17_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRCH-ACV-9B-IgG_S17_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31996759
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT675520.21112138263753524No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT664910.20780542179287595TruSeq Adapter, Index 13 (97% over 38bp)
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG618670.1933539581305719No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG528350.16512609917773233No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC485020.15158410262739422No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC469050.14659297211945746No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC452450.1414049466697549No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC452150.14131118717367594No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCC440520.13767644404234816No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCC429800.13432610471579323No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCC427130.13349164520069048No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC423560.13237590719735084No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC422350.13199774389649901No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC416010.13001629321269695No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC407850.12746603491934919No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC403140.12599401083090947No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCC400630.12520955638038214No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC399450.12484076902913824No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC389140.12161856768055788No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC382070.11940896888963035No Hit
CAAGGTCGCTGTTCAATACATCGGACGCCGATTACGTGTCGCGCGTAGCC371610.11613988779301053No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC359830.11245826491364327No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCC359530.11236450541756433No Hit
CAAGGTCGCTGTTCAATACATCGGACGATCGTACGATAGCGTACCGAGCC357870.11184570287259407No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC350380.10950484078715599No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA344460.10765465339786445No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCC341540.10674206096936256No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC341350.1066826799551792No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC340590.10644515589844585No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT339590.10613262424484929TruSeq Adapter, Index 13 (97% over 38bp)
CAAGGTCGCTGTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCC328390.10263226972456803No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATCGGTATCGGTACGCGCGCC320650.10021327472573081No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)