FastQCFastQC Report
Mon 19 Jun 2023
RCH-ACV-2C-H3K4me3_S22_L004_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRCH-ACV-2C-H3K4me3_S22_L004_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30395476
Sequences flagged as poor quality0
Sequence length50-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA6516882.144029591772144No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC4589551.5099450984087237No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC4449101.4637375641032897No Hit
TACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGT1628030.5356158923123955No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG1368680.45029069457573223No Hit
CGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTAG1046790.34439006646910214No Hit
TGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATA1016530.3344346375756708No Hit
ATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAAT947620.311763500594628No Hit
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT591660.1946539675838602No Hit
ACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAGGGAGTAA577550.19001182939197925No Hit
AGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGCG577440.18997563979586962No Hit
ACATCGGATCGACGCGTAAACGGTACGTCGGCCTGGAGACTAGGGAGTAA420960.13849429434827737No Hit
TAGCTCAATTGGTCGTAGCAAGCTCTAGCACCGCTTAAACGCACGTACGC351530.11565207927653444No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG349450.11496776691373414No Hit
AGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCTG325460.10707514499855175No Hit
AGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCCTG309340.10177172418684938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)