FastQCFastQC Report
Mon 19 Jun 2023
RCH-ACV-9B-IgG_S17_L004_R1_001.trim.paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRCH-ACV-9B-IgG_S17_L004_R1_001.trim.paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30592417
Sequences flagged as poor quality0
Sequence length50-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTT658350.21520038773007047No Hit
GATAGACAGCTGCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCG603350.19722207630734112No Hit
ATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCG517550.16917591048788333No Hit
GCTGAACCAATGGGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTC473910.15491093756992133No Hit
CAAGGTCGCTGTTCAATACATCGGACGCATATCGCGTCGTACGACCGGCC462280.15110934189998781No Hit
CAAGGTCGCTGTTCAATACATCGGATTATCGCGTCGCGACGGACGTAGCC447350.14622904754469057No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCCGATCGATCGTCGGGCC445730.14569950455369382No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATACGACCGCGTTACGCGGCC435110.14222805605715952No Hit
CAAGGTCGCTGTTCAATACATCGGATTCGCGCGTAACGACGTACCGTGCC424360.1387141133699897No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGAAATTCGTATACGCGTCGGCC421520.13778577874379785No Hit
CAAGGTCGCTGTTCAATACATCGGACGACGTTAACGCGTTTCGTACGGCC417680.13653056572810182No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGACTATCGCGCGTAACGCGGCC416370.13610235503785137No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGATAAGACGCGTAACGGCC410410.13415415983640652No Hit
CAAGGTCGCTGTTCAATACATCGGATTTCGACGCGTCGATTCGGCGAGCC402800.1316666152922798No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGATACGACTCGTTCGTCGGCC397820.130038760912549No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTATAGTCCGAGCGTAGCC395690.12934250994290514No Hit
CAAGGTCGCTGTTCAATACATCGGATATCGCGCGAAACGACCGTTCGGCC394380.12891429925265466No Hit
CAAGGTCGCTGTTCAATACATCGGACCGCGCGTAATGCGCGACGTTAGCC383520.1253643999426394No Hit
CAAGGTCGCTGTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCC376790.12316450838127631No Hit
CAAGGTCGCTGTTCAATACATCGGACGCCGATTACGTGTCGCGCGTAGCC366560.11982054245664865No Hit
CAAGGTCGCTGTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCC355010.1160450970578755No Hit
CAAGGTCGCTGTTCAATACATCGGACGATCGTACGATAGCGTACCGAGCC353850.11566591812605066No Hit
CAAGGTCGCTGTTCAATACATCGGAACGCGAATCGTCGACGCGTATAGCC345980.11309338520065282No Hit
CAAGGTCGCTGTTCAATACATCGGACGATTCGACGGTCGCGACCGTAGCC343650.1123317585531081No Hit
ACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATTGGTCGTA337520.11032799402544755No Hit
CAAGGTCGCTGTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCC337120.11019724266964588No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATATCACTCGACGCGATAGCC337080.11018416753406572No Hit
CAAGGTCGCTGTTCAATACATCGGACGTTCGAACGTTCGTCGACGATGCC336070.10985402036066651No Hit
CAAGGTCGCTGTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCC323570.10576804049186439No Hit
CAAGGTCGCTGTTCAATACATCGGACGCGATCGGTATCGGTACGCGCGCC315680.10318896999867648No Hit
CAAGGTCGCTGTTCAATACATCGGAATCGTACCGCGCGTATCGGTCGGCC313970.10263000795262434No Hit
CAAGGTCGCTGTTCAATACATCGGAGTGATATCGCGTTAACGTCGCGGCC312770.10223775388521934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)