[General] title = NSD_RS_20230622 logfile = rnaseq.log seqproject = GE-6783 # If specified, this will be added to each submitted job as a comment label = JL # Link to project page # project = # List the different experimental conditions. Each condition may include multiple # biological replicates. For example, a wildtype and two different knockouts: conditions = WT-NSD2i-0, WT-NSD2i-1, Mut-NSD2i-0, Mut-NSD2i-1 # Specify the differential analysis contrasts, separating each pair of samples with ^. contrasts = Mut-NSD2i-1^Mut-NSD2i-0, WT-NSD2i-1^WT-NSD2i-0, Mut-NSD2i-1^WT-NSD2i-0 steps = rnasamples, fastqcount.1, trim, fastqcount.2, startx, merge, bamcount, bamcov, rsemquant, deseq, mats, simplediff, bamtobigwigfast, multiqc, methods, hub [Diff] fdr = 0.05 fc = 1 [Hub] # Options in this section are used when generating a UCSC Hub hubname = NSD_RS_20230622 shortLabel = NSD_RS_20230622 longLabel = NSD_RS_20230622 genome = hg38 sizes = hg38.chrom.sizes dirname = hub email = your@email.com url = https://lichtlab.cancer.ufl.edu/reports/NSD2/ [Include] # All genome files are listed in a separate conf file genome = /data/reference/icbr/GRCh38/GENOMEFILES # If fastqs are listed in a separate file, use this fastqs = fastqs.conf