LichtLab Sequencing - run details
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Project
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Evans MDS
H2B - E76K
LeleLab collaboration
Melanoma
NSD2 - E1099K
Other
SETD2 inhibitor
UTX - KDM6A
Assay
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RNA-Seq
ChIP-Seq
CUT&RUN
ATAC-Seq
scRNA-Seq
CRISPR
Title
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Organism / cell line
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ICBR number (if known)
Owner
Notes
CRISPR screen with brunello sgRNA library on Uv. melanoma 92.1 cells +/- PKC inhibitor
Samples
Enter sample names, one per row. If possible, use the format COND_SMP, where COND is an experimental condition and SMP is a unique sample name.
92.1_P0 92.1_control_P3 92.1_control_P6 92.1_control_P8 92.1_PKCi_P2_A 92.1_PKCi_P5_A 92.1_PKCi_P7_A 92.1_PKCi_P2_B 92.1_PKCi_P5_B 92.1_PKCi_P7_B
Contrasts
Enter contrasts, one per row. A contrast consists of two sample name separated by spaces, as in "Test Control".
Please run the QC for CRISPR screens and then prepare a read count table for the sgRNAs in each sample. We used the brunello library for this screen.
Options
ERCC spike-ins
Dmel spike-ins
Ecoli spike-ins
Analyzed
Analysis notes