LichtLab Sequencing - run details
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Project
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Evans MDS
H2B - E76K
LeleLab collaboration
Melanoma
NSD2 - E1099K
Other
SETD2 inhibitor
UTX - KDM6A
Assay
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RNA-Seq
ChIP-Seq
CUT&RUN
ATAC-Seq
scRNA-Seq
CRISPR
Title
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Organism / cell line
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ICBR number (if known)
Owner
Notes
CRISPR screen with brunello sgRNA library on Uv. melanoma Mel202 cells +/- PKC inhibitor
Samples
Enter sample names, one per row. If possible, use the format COND_SMP, where COND is an experimental condition and SMP is a unique sample name.
Brunello plasmid Brunello library virus Mel202_P0 Mel202_control_P3 Mel202_control_P5 Mel202_control_P7 Mel202_PKCi_P3 Mel202_PKCi_P5 Mel202_PKCi_P7
Contrasts
Enter contrasts, one per row. A contrast consists of two sample name separated by spaces, as in "Test Control".
Please run the CRIPS QC pipeline and then create a read counts table for the sgRNAs in the brunello library
Options
ERCC spike-ins
Dmel spike-ins
Ecoli spike-ins
Analyzed
Analysis notes